New defined HLA class I epitopes predicted by computer analysis are recognized by specific CTL in patients post allogeneic HSCT.

IFN-γ staining of PBMC from, patient No. 17 (AML, CR), 672 days post transplantation (A), patient No. 8 (AML, CR), 1035 days post transplantation (B) Cells were stimulated with 10μg/ml of the indicated peptides. Gates were set on lymphocytes by forward/side scattering (R1) and on CD3⁺/CD8⁺ cells (R2). Percentage numbers show peptide-specific CD3+/CD8+ T-cells from all CD3⁺/CD8⁺ T-cells.