RBC p50 (mm Hg) and 2,3-DPG concentration (mmol/g Hb) for paired Rejuvenated and Control groups after storage for 3-6, 7, 14, 21, 28, and 35 days. 2,3-DPG and p50 values were significantly different between groups at each time-point (p < 0.001, t-test).