(A) The top line shows the germline sequence of a section of the V segment. The clonotype in the diagnostic sample has 3 SHMs shown in the section. The clonotype in the follow up sample has acquired an additional SHM. B) Germline V and J sequences are shown on top with the boxed sequences present in the clonotypes and the unboxed bases representing the sequences that were deleted during the VDJ recombination to form the clonotypes. The four arrows denote SHMs that have occurred to generate an inferred but unobserved clonotype. One additional mutation occurs to form the clone seen at diagnosis, while three different mutations appear in the clonotype seen in the follow up sample.