Acute MCMV infection promotes differentiation of inflammatory Ly6C^HImonocytes. (A) Kinetics of circulating CD11b⁺Ly6C^HI cells in ECDI-SP–treated, either uninfected or Δm157-infected (on day 0), transplant recipients. Total live CD11b⁺Ly6C^HI cells were enumerated by FACS in 50 µL of blood drawn on the indicated days. (B) Quantitative analysis of total CD11b⁺Ly6C^HI cells in 50 µL of blood collected on day 10 posttransplantation from recipients of the indicated groups. (C) Representative FACS plots demonstrating the expression pattern of CD115 and CD11c on circulating Ly6C^HI cells from the indicated groups on day 10 posttransplantation. Data shown in panels A-C were from 2 to 3 independent experiments with a total of 4 to 6 mice in each group. *P < .05. (D) Representative FACS plot demonstrating graft-infiltrating Ly6C^HI cells (gated on total graft-infiltrating live CD11b⁺ cells; day 10 posttransplant). Scatter graph showing quantitative analysis of the number of graft-infiltrating Ly6C^HI cells (N = 6 in each group). (E) Representative FACS plots demonstrating phenotypic expression of CD115 and CD11c on graft-infiltrating Ly6C^HI cells shown in panel D. (F) Representative FACS plots demonstrating expression of intracellular IL-12p40, surface CD86, and MHC II from graft-infiltrating Ly6C^HI cells shown in panel D. Scatter graphs showing quantitative analysis of MFIs of the indicated markers. Data shown in panels D-F were obtained from 3 independent experiments with a total of 4 to 6 mice in each group. Data were presented as mean ± SD. *P < .05.