American Society of Hematology

Figure 2.

$Figure 2. Normal steady-state early B-cell development in Tet2-deficent mice. (A) qRT-PCR analysis transcription levels of the 3 murine Tet genes normalized by Abl1 expression. Results are the average of 3 independent experiments performed in duplicate. (B) Bone marrow B-cell development analysis. Total BM cells were analyzed for B220 and IgM expression. Immature B cells B220lowIgM+ and mature B cells B220+IgM+ are indicated. Then CD43 expression was evaluated in gated B220+IgM− cells to define pre-B (CD43−) and pro-B (CD43+) populations; finally, the more immature Hardy fractions (Fr.A CD24−Bp1−; Fr.B: CD24+Bp1−; and Fr.CC′: CD24+Bp1+) were analyzed in the pro-B-cell gate. The genotypes of the analyzed mice are indicated on the left side. (C) Average percentage of cell populations. (Left) Total B220+ cells (each dot represents an independent mouse). (Middle) Average percentage of the indicated population in the B-cell compartment for each genotype. (Right) Hardy fraction percentage inside the pro-B-cell compartment. Genotypes are indicated (Tet2+/+ mice, n = 6; Tet2−/− mice, n = 8). Im-B, immature B cells B220lowIgM+; Mat-B, mature B cells B220+IgM+.$

Normal steady-state early B-cell development in Tet2-deficent mice. (A) qRT-PCR analysis transcription levels of the 3 murine Tet genes normalized by Abl1 expression. Results are the average of 3 independent experiments performed in duplicate. (B) Bone marrow B-cell development analysis. Total BM cells were analyzed for B220 and IgM expression. Immature B cells B220^lowIgM⁺ and mature B cells B220⁺IgM⁺ are indicated. Then CD43 expression was evaluated in gated B220⁺IgM⁻ cells to define pre-B (CD43⁻) and pro-B (CD43⁺) populations; finally, the more immature Hardy fractions (Fr.A CD24⁻Bp1⁻; Fr.B: CD24⁺Bp1⁻; and Fr.CC′: CD24⁺Bp1⁺) were analyzed in the pro-B-cell gate. The genotypes of the analyzed mice are indicated on the left side. (C) Average percentage of cell populations. (Left) Total B220⁺ cells (each dot represents an independent mouse). (Middle) Average percentage of the indicated population in the B-cell compartment for each genotype. (Right) Hardy fraction percentage inside the pro-B-cell compartment. Genotypes are indicated (Tet2^+/+ mice, n = 6; Tet2^−/− mice, n = 8). Im-B, immature B cells B220^lowIgM⁺; Mat-B, mature B cells B220⁺IgM⁺.

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