Figure 6.
![Figure 6. Human endothelial heparanase expression in response to p53 modulation. Results after quantitative analysis of (A) tumor suppressor p53 (TP53), (B) heparanase (HPSE), (C) EGR1, (D) PTEN, and (E) TF protein expression in HUVECs following incubation with doxorubicin (500 nM for 24 hours), pifithrin-α (20 µM for 24 hours), or vehicle alone (saline or dimethyl sulfoxide [DMSO] = control). Quantitative analysis of findings in n = 4 biological replicates. *P < .05, **P < .01, and ****P < .0001. (F) Representative western blot membranes from the same experiment are shown. GAPDH was used as control for equal protein loading.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/11/10.1182_bloodadvances.2017014050/3/advances014050f6.jpeg?Expires=1724941810&Signature=dcIlDX2XTtx3unpDPrLeQyuk14pAJPpo271w9alWMrhc96k5vbvvgnICb3I-gLD48qOLU6CgyChpp65i0fLziKq1CU2AAd2u2UtFNKFAZcOFCTp9mzdc7HGmwqwQnrwQOSgwirpddVtdIKToFzoKMrSHdTdYEsP0cssvlB6kyOs~K1dnGzbJRdXnngowERuBNzQxpL4WUgzlbQXCO7jzI8VMdl5M-rRLEwc0jJ8N1hJgz2J3rjW2g7Ua3tSv~32bQLFV--GoTBoJ0r4GmrJx6vr9p1tZAWeQgT4aPT42EVZLPO96cUXj2v0NbJ6MN3h4xsySVeYKk7IM9H0SRWVN6Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Human endothelial heparanase expression in response to p53 modulation. Results after quantitative analysis of (A) tumor suppressor p53 (TP53), (B) heparanase (HPSE), (C) EGR1, (D) PTEN, and (E) TF protein expression in HUVECs following incubation with doxorubicin (500 nM for 24 hours), pifithrin-α (20 µM for 24 hours), or vehicle alone (saline or dimethyl sulfoxide [DMSO] = control). Quantitative analysis of findings in n = 4 biological replicates. *P < .05, **P < .01, and ****P < .0001. (F) Representative western blot membranes from the same experiment are shown. GAPDH was used as control for equal protein loading.
