Thrombus formation and resolution over 3 weeks. (A) Percentage of adult and aged End.p53-WT and End.p53-KO mice, in which thrombosis after subtotal IVC ligation was observed or not. The absolute numbers per group and category (“thrombus” and “no thrombus”) are also given. Results were analyzed using χ2 test. *P < .05, ***P < .001, and ****P < .0001. (B) Thrombus size at day 1 after subtotal IVC ligation. Only mice that formed a thrombus are shown. Graphs show individual values as well as the mean ± SEM and were analyzed using 1-way ANOVA. *P < .05. (C) Representative ultrasound images of venous thrombi (encircled by broken white lines) at day 1 after subtotal IVC ligation. The calculated thrombus area is also shown. (D) Venous thrombus resolution in mice that formed a thrombus. Results are expressed as the percentage change vs thrombus size at day 1 (set at 100%) and were analyzed using 2-way ANOVA. ***P < .001 vs day 1 and #P < .05 vs adult End.p53-WT mice. (E) Serial cross sections through the thrombosed IVC segment of adult and aged End.p53-WT or End.p53-KO mice were examined 21 days after surgery and the thrombus cross-sectional area determined. Only mice that formed a thrombus are shown. *P < .05. (F) Representative Carstairs-stained cross sections of thrombosed IVC segments are shown. Scale bars represent 200 µm.
