IL-2 supports in vivo function of BC133-redirected T cells. Female NSG mice were implanted IV with 1 million MOLM13 AML cells. Tumor growth was monitored by bioluminescence imaging (A) and expressed as total flux in p/s (B). Starting 7 days after leukemia implantation, ATCs (5 million-10 million/dose) were injected once per week for 3 weeks. BC133 (10 μg) was administered 1 day before and 1 day after each T-cell administration. One group of ATC/BC133 recipients received IL-2 (1000 IU subcutaneously) 2 to 3 times per week and the other group did not receive any IL-2.