DCA inhibits experimental arterial thrombosis without altering tail bleeding time. (A) The tail-transection bleeding time was determined as the time taken for the initial cessation of bleeding after transection in vehicle or DCA-treated group. Each symbol represents a single mouse. Horizontal bar shows the mean of each group ± SEM, n = 15 per group. (B) Aggregation induced by collagen (0.5 µg/mL) in PRP from mice treated with DCA or vehicle. Upper panel denotes the representative aggregation curves (blue, vehicle; black, DCA). Values are represented as mean ± SEM, with n = 4 mice per group. (C) The left panel shows representative microphotographs (4× objective), and right panel shows time to occlusive thrombus in the FeCl3-induced carotid artery injury model. Each symbol represents a single mouse. Horizontal bars show the mean of each group ± SEM, with n = 8 to 10 per group. (D) The left panel shows representative microphotographs (10× objective), and right panel shows time mean fluorescence intensity in the laser-induced mesentery artery injury model. N = 10 to 12 vessels from 4 mice per group. *P < .05 compared with vehicle control. Platelets were labeled with calcein green. White line delineates vessel.
