DCA inhibits glucose uptake in agonist-induced platelet activation. Measurement of 2-DG uptake in washed human (A) and mouse (B) platelets with a luminescence-based assay kit. Luminescence (produced by 2DG6P) was monitored in resting and platelets response to suboptimal dose of collagen, convulxin, thrombin, and ADP. For mouse studies, washed platelets were pooled from 3 to 4 mice (n = 5 individual experiments per group). For human studies, washed platelets are from the individual donors (n = 5 per group). Values are mean ± SEM. *P < .05 vs unstimulated platelets; #P < .05 vs control (vehicle).