Figure 3.
Figure 3. The impact of deleting RGS10 on Ca++ mobilization and myosin light chain. (A-B) Ca++ mobilization. Platelets were stimulated with AYPGKF, ADP, or U46619 at the concentrations indicated in the absence of extracellular Ca++. (A) Representative measurements. (B) Summary of the results of 4 experiments (mean ± SEM). (C) Myosin light chain (MLC) phosphorylation. Washed platelets were incubated for 3 minutes with 350 µM AYPGKF to activate PAR-4. Lysates were probed with an antibody specific for MLC phosphorylated on Thr18 and Ser19 before reprobing with anti-MLC. Data are mean ± SEM. N = 4.

The impact of deleting RGS10 on Ca++mobilization and myosin light chain. (A-B) Ca++ mobilization. Platelets were stimulated with AYPGKF, ADP, or U46619 at the concentrations indicated in the absence of extracellular Ca++. (A) Representative measurements. (B) Summary of the results of 4 experiments (mean ± SEM). (C) Myosin light chain (MLC) phosphorylation. Washed platelets were incubated for 3 minutes with 350 µM AYPGKF to activate PAR-4. Lysates were probed with an antibody specific for MLC phosphorylated on Thr18 and Ser19 before reprobing with anti-MLC. Data are mean ± SEM. N = 4.

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