Figure 1.
Akt activation fully mimics IL-7-induced T-ALL cell growth and cell cycle progression but does not completely recapitulate the effects on cell viability. HPB-ALL cells were stably transduced with retroviral vectors driving the expression of constitutively active, myristoylated-Akt (myrAkt), or empty control (Empty). (A) Transduced, regularly cultured HPB-ALL cells collected for immunoblot analysis of Akt expression (Akt) and PI3K/Akt, JAK/STAT5, and MEK/extracellular signal-regulated kinase (ERK) pathway activation (P-Akt, P-STAT5, and P-ERK1/2, respectively). Starved transduced HPB-ALL cells were stimulated or not with IL-7 (20 ng/mL) and were assessed by flow cytometry analysis of cell growth at 96 hours (B), cell cycle at 72 hours (C) and viability at 96 hours (D).

Akt activation fully mimics IL-7-induced T-ALL cell growth and cell cycle progression but does not completely recapitulate the effects on cell viability. HPB-ALL cells were stably transduced with retroviral vectors driving the expression of constitutively active, myristoylated-Akt (myrAkt), or empty control (Empty). (A) Transduced, regularly cultured HPB-ALL cells collected for immunoblot analysis of Akt expression (Akt) and PI3K/Akt, JAK/STAT5, and MEK/extracellular signal-regulated kinase (ERK) pathway activation (P-Akt, P-STAT5, and P-ERK1/2, respectively). Starved transduced HPB-ALL cells were stimulated or not with IL-7 (20 ng/mL) and were assessed by flow cytometry analysis of cell growth at 96 hours (B), cell cycle at 72 hours (C) and viability at 96 hours (D).

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