IV-injected Co-HP inhibited rhodocytin-induced platelet aggregation. Five minutes (A) or 48 hours (B) after IV injection of 1% DMSO or Co-HP (estimated final concentration of 10 μg/mL [15 μM]), the mice were euthanized and the blood was drawn from the IVC with 5% final concentration of ACD. PRP was stimulated by the indicated concentrations of rhodocytin, collagen, or PAR4-AP (thrombin receptor activator peptide) and platelet aggregation was monitored for 10 minutes using a platelet aggregometer.