CD73 is important for elevation of plasma adenosine, erythrocyte p-AMPK, erythrocyte 2,3-BPG mutase activity, and erythrocyte 2,3-BPG in both SCD mice and SCD patients. (A) Erythrocyte p-AMPK, (B) erythrocyte 2,3-BPG mutase activity, (C) erythrocyte 2,3-BPG level, and (D) hemoglobin oxygen release capacity (P50) in WT BMT, SCD BMT, and SCD/CD73^−/− BMT mice. (E) Plasma CD73 activity, (F) plasma adenosine level, (G) erythrocyte p-AMPK levels quantified by ELISA, (H) erythrocyte 2,3-BPG mutase activity, and (I) 2,3-BPG level in normal individuals and sickle cell disease patients. Data are expressed as mean ± SEM. n = 5 for each group; *P < .05 vs control, **P < .05 vs SCD, Student t test.