Genetic deletion of CD73 in SCD mice decreases hemolysis and increases erythrocyte lifespan by attenuating plasma CD73 activity and plasma adenosine level. (A-B) Plasma adenosine level and plasma CD73 activity level in WT and SCD mice. (C) Correlation of plasma CD73 activity and plasma adenosine level in SCD mice. (D) Plasma CD73 activity and (E) plasma adenosine level in WT BMT mice, SCD BMT mice, and SCD/CD73^−/− BMT mice. (F) Representative image of sickled erythrocytes and the percentage of sickled erythrocytes in SCD BMT mice and SCD/CD73^−/− BMT mice (magnification ×400). (G) Lifespan of erythrocytes in SCD BMT and SCD/CD73^−/−BMT mice. (H) Plasma total hemoglobin and (I) plasma bilirubin in WT BMT mice, SCD BMT mice, and SCD/CD73^−/− BMT mice. Data are expressed as mean ± SEM. n = 5 for each group; *P < .05 vs WT, **P < .05 vs SCD, Student t test.