CD8_CXCR5+ICOS+gene expression profile. (A) Venn diagram allowing the retrieval of shared downregulated genes between the CD8_CXCR5+ICOS+ (blue) and T_FH (red) signatures. (B) Venn diagram allowing the retrieval of shared upregulated genes between the CD8_CXCR5+ICOS+ (blue) and T_FH (red) signatures. (C) Gene set enrichment score curves for T_FH signature in CD8_CXCR5+ICOS+ and CD8_{CXCR5−ICOS−} cell subsets. Vertical black lines indicate the position of genes included in the specific T_FH signature. (D) Heatmap representation of the 406 most variably expressed genes of the T_FH signature for memory CD4⁺ T cells (Mem T), T_FH cells, CD8_CXCR5+ICOS+ cells (CD8 T NEG), and CD8_CXCR5+ICOS+ cells (CD8 T POS).