The Rap1 GEF RasGRP2 is required for the enhanced migration of MEC1-CD38H cells. MEC1-GFP and MEC1-CD38H cells were transfected with siRNAs targeting RasGRP2 or RasGRP3. (A) Representative blots of RasGRP2 and RasGRP3 expression levels. (B) Migration of MEC1-GFP and MEC1-CD38H cells through transwell filters upon RasGRP2 and RasGRP3 depletion. The migration index was obtained by normalizing the values to the MEC1-GFP control cells. Data shown are the mean of 3 independent experiments ± SEM. *P < .05, determined by 2-tailed Student t test. (C) Representative blot (left) and quantification (right) of active Rap1-GTP levels in MEC1-GFP and MEC1-CD38H cells after RasGRP2 depletion. Graph shows the mean of 3 independent experiments ± SEM. *P < .05; **P < .01 determined by 2-tailed Student t test.