In vitro expression of recombinant VWF mRNA. (A) SNV c.2365A>G. (B) SNV c.2385T>C. (C) Both SNVs in cis. Genotypes compared using an unpaired Student t test (*P < .05; **P < .01; ****P < .0001). Mean values for n = 3 triplicate measurements are shown (bars indicate standard error of the mean).