In vitro expression of recombinant VWF protein. (A) SNV c.2365A>G. (B) SNV c.2385T>C. (C) Both SNVs in cis. Genotypes compared using a 2-way ANOVA with the Tukey multiple comparisons test (*P < .05; **P < .01; ***P < .001; ****P < .0001). Mean values for n = 3 triplicate measurements are shown (bars indicate standard error of the mean).