Detection of KRAS^G12Dmutation and inv(16) in sorted blasts and eosinophils. (A) Dot plot of bone marrow cells (yellow: eosinophils; red: blasts; blue: lymphocytes; pink: monocytes; green: neutrophils). (B) Sorted blasts (CD45^dimCD34⁺CD15⁻CD117⁺SSC^lo). (C) Sorted eosinophils (CD45⁺CD34⁻CD117⁻CD15⁺SSC^hi). FISH studies using CBFB break-apart probes (Abbott Molecular, Des Plaines, IL) showing a split signal pattern of CBFB probes (ie, inv(16)), in (D) sorted blasts and in (E) eosinophils. Detection of KRAS^G12D in (F) sorted blasts, (G) eosinophils, and (H) FFPE adrenal tissue by ddPCR. Blue dots represent mutant droplets; green dots represent wild-type droplets, and red or gray dots represent empty droplets. SSC-H, side-scatter height.