Figure 5.
![Figure 5. 161533 TriKE is superior to 1633 BiKE to restore NK-cell function and proliferation in patients with MDS. (A) Cryopreserved PBMCs from MDS patients (n = 14) and HDs (n = 7) were rested overnight and stained, and NK cell frequencies were determined by flow cytometry. (B-C) Cryopreserved PBMCs from MDS patients (B) or HDs (C) were activated overnight with IL-15 (equal molar concentration to the IL-15 in TriKE [50 nM], BiKE [50 nM], or TriKE [50 nM]). Target cells were then added 6 hours prior to staining. NK cell degranulation (CD107a), IFN-γ production, and proliferation (Ki67) were evaluated. Data are shown as mean ± SEM, and statistical analyses were performed using paired Student t test.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/12/10.1182_bloodadvances.2017012369/3/advances012369f5.jpeg?Expires=1724157652&Signature=T61BFEq6bLZaoQCjKmc0mdyQ0ZInDWgqQCu-ynrwQ2DV-tHAseWj9PVuSvALmIE4JgyizaTRAg82xtkgc0cwCeCLTpMDuy6cCkJ0oI05bzYb9dyvZ6k8oegEBfMF7dQgWR4YjXoQqE~fvrlSA9DxIW1IsocFAYFmenKfBm3C1IdsGjMwNg19rvtKpintD3w870KNKGPKvkKOOBPaWl-mmpzlBpp5kuD~Bl4Cc~pyMmTG4fP8YTuzITCvU9NiyKCugZi-FGaBUJfltAcHsKAZ1WeVAZhTgffMiu4VyNCpn0PYRQAINDidZB-l1HxXFrQY1PxSPWJVPACprps2Up1DQg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
161533 TriKE is superior to 1633 BiKE to restore NK-cell function and proliferation in patients with MDS. (A) Cryopreserved PBMCs from MDS patients (n = 14) and HDs (n = 7) were rested overnight and stained, and NK cell frequencies were determined by flow cytometry. (B-C) Cryopreserved PBMCs from MDS patients (B) or HDs (C) were activated overnight with IL-15 (equal molar concentration to the IL-15 in TriKE [50 nM], BiKE [50 nM], or TriKE [50 nM]). Target cells were then added 6 hours prior to staining. NK cell degranulation (CD107a), IFN-γ production, and proliferation (Ki67) were evaluated. Data are shown as mean ± SEM, and statistical analyses were performed using paired Student t test.
