Figure 4.
![Figure 4. Crosslinked CD16 enhances the IL-15–induced phosphor-STAT5 signal. Isolated NK cells (n = 9; by negative depletion) from HDs were stimulated with IL-15 (equal molar concentration to the IL-15 in TriKE [50 nM], BiKE [50 nM], BiKE + IL-15 [both 50 nM], or TriKE [50 nM]) with or without HL60 targets for 25 minutes before fixation and STAT5 phosphorylation analysis. Symbols represent distinct individuals, and statistical analysis was performed using a paired Student t test.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/12/10.1182_bloodadvances.2017012369/3/advances012369f4.jpeg?Expires=1724158245&Signature=TgKnXHSmHvx8LvrlmCOn4D6Q7jJrgSW5F-rmBjr9hcIReyF3fCceKlgTBCTuP6IdLl-u4d0GxtYkezlm5vHX6q522L8AELG275LfN~rd3noS5QtN2A9TdlqR2S9YOS3d1dK5s83RkIqHwyZwf5NgFT6RFNqNZ6mi5Irb62tFy7MvKHbtBta2bSrkifhOAwYAuF~~1oJKGYKVn5-qAAh4JF4~kOYVIgYvEgjgFn8DmTdlk33QBbiRMJhULIYLaAEuKM44EJc4q6cWKKBVde8NEM8MOEX86gOEFM8pOIsV8wOWvyzCZ1w44AUnRRn9iCk6~-fBKZWsBrPxZvT1Ty8vOA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Crosslinked CD16 enhances the IL-15–induced phosphor-STAT5 signal. Isolated NK cells (n = 9; by negative depletion) from HDs were stimulated with IL-15 (equal molar concentration to the IL-15 in TriKE [50 nM], BiKE [50 nM], BiKE + IL-15 [both 50 nM], or TriKE [50 nM]) with or without HL60 targets for 25 minutes before fixation and STAT5 phosphorylation analysis. Symbols represent distinct individuals, and statistical analysis was performed using a paired Student t test.
