Figure 4.
![Figure 4. VWF/p.V1316M affects platelet granule release. (A) αIIbβ3 integrin activation measured with JON/A-PE, added 10 minutes after stimulation with ADP (10 μM) and U46619 (5 μM). MFI in stimulated WT platelets (red bars) set as 100%. Red bars, VWD2B(p.V1316M) platelets. (B) Quantification of secreted [3H]serotonin from δ-granules of washed WT and VWD2B(p.V1316M) platelets (red and blue bars, respectively), stimulated or not with PAR4p (1 mM). (C) P-selectin surface expression on PAR4p-stimulated WT and VWD2B(p.V1316M) platelets (red and blue bars, respectively). Data are shown as mean ± SD of 3-17 mice per group. **P ≤ .01; ***P ≤ .001.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/12/10.1182_bloodadvances.2017014290/3/advances014290f4.jpeg?Expires=1724952020&Signature=AIUNzJmG1m-V9LcOXepPUWos6M5u2FKY~zsnfT7iNjWkYRM8TAlFddYe-TB4wXCmlWfvgeyIH6KQzzYo5l-0yI8UhJybgsm988BJ8xjYcmZLFzmelqud1G~aCeOrWTmBnX2LgJE4EFjCuj8bxaPC9DoCD3Gi5tFIyy1nOQWxLdUieHUx2dycP~oien9THnXVQgZyUO5bhCmdl85DrkoV35famRyk9PZGmRMw2nnUWA~ZjBA1ex6EsEowpdixGIoRWerl4iAXoWk8sCpol817-Q-cjzUvP7zmIqE2zGp9BKndih1RT1pdTjzLNpPNvZ-MYaWpGtqeN4vy7zyUs5EkjQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
VWF/p.V1316M affects platelet granule release. (A) αIIbβ3 integrin activation measured with JON/A-PE, added 10 minutes after stimulation with ADP (10 μM) and U46619 (5 μM). MFI in stimulated WT platelets (red bars) set as 100%. Red bars, VWD2B(p.V1316M) platelets. (B) Quantification of secreted [3H]serotonin from δ-granules of washed WT and VWD2B(p.V1316M) platelets (red and blue bars, respectively), stimulated or not with PAR4p (1 mM). (C) P-selectin surface expression on PAR4p-stimulated WT and VWD2B(p.V1316M) platelets (red and blue bars, respectively). Data are shown as mean ± SD of 3-17 mice per group. **P ≤ .01; ***P ≤ .001.
