FXII and FXI functional inhibitor(s) are likely to be responsible for coagulopathy in AD samples. (A) Clotting start time of NPP and NPP mixed with ND or AD plasma was assessed via aPTT. (B) Plasma deficient in FIX, FX, FXI, or FXII was mixed with ND or AD plasma (9:1), and clotting start time was assessed via aPTT. Data are represented as the fold change over the average of the ND sample mixed with each factor-depleted plasma. Plasma protein levels of FXI (C) and FXII (D). For NPP-mixing experiments, 1-way ANOVA was performed with Tukey’s post hoc analysis. For each factor-depleted plasma experiment, the unpaired Student t test was performed between ND and AD with Welch’s correction. *P < .05, **P < .01, ***P < .001.