Figure 4.
![Figure 4. Red pulp macrophages exhibit an expression pattern distinct from monocytes and monocyte-derived macrophages. Flow cytometry stainings on nonautofluorescent CD163intCD14high spleen monocytes, CD14high blood monocytes gated on FSC/SSC patterns, autofluorescent CD163highCD14low red pulp macrophages from spleen, and M-CSF and GM-CSF cultured monocyte-derived macrophages, (A) CD11a, (B) CD11b, (C) CD11c, (D) CD18, (E) CD89, (F) CD200R, (G) HLA-DR, (H) gp91PHOX (reduced NAD phosphate [NADPH] oxidase complex), (I) TLR4, and (J) IgG (polyclonal goat anti-human IgG). Mean ± SEM of n ≥ 8 are shown for each group.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/8/10.1182_bloodadvances.2017015008/3/advances015008f4.jpeg?Expires=1723413693&Signature=CiIXd70VHEh0ujRQ~K-3U-bHvR9Lv7j8BlA7HkYurlA~PJ19K04ONTff0E5dW41WztzE0znlh5O6GbgfS04G8KOr2NGK3K45I0PkLfG368ylcijMMVhVPAUz9ApmwDA~2DkUFXf15osbF4Wa~atZl5u0FO31ZiTGMvXxRPIFKAeomZlf4Fe~BoaeoskEM5UAoaoBrkAT6qhV~dCUtNioj5H~PCmxDPY6RYwkINU9iOEjX183UZ4Vhzrxf1IxOx6Qe8SwjvkK6fzmQRdoVxOp20OdatMAKhU9SVvNCW3xImenSEf80qRxyMvrtXE11NuAGDmqdkBDGaES5rRRGpcM2A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Red pulp macrophages exhibit an expression pattern distinct from monocytes and monocyte-derived macrophages. Flow cytometry stainings on nonautofluorescent CD163intCD14high spleen monocytes, CD14high blood monocytes gated on FSC/SSC patterns, autofluorescent CD163highCD14low red pulp macrophages from spleen, and M-CSF and GM-CSF cultured monocyte-derived macrophages, (A) CD11a, (B) CD11b, (C) CD11c, (D) CD18, (E) CD89, (F) CD200R, (G) HLA-DR, (H) gp91PHOX (reduced NAD phosphate [NADPH] oxidase complex), (I) TLR4, and (J) IgG (polyclonal goat anti-human IgG). Mean ± SEM of n ≥ 8 are shown for each group.
