Immunofluorescence of spleen tissue shows FcγR expression pattern of splenic macrophages. Immunofluorescent costainings of (A) CD163, FcγRIII, and CD19; (B) CD169, FcγRIII, and CD19; (C) CD163, FcγRI, and FcγRIIb; (D) CD169, FcγRI, and FcγRIIb; (E) CD163 and FcγRIIa+b; and (F) CD169 and FcγRIIa+b. Original magnification ×10. CD163 marks the red pulp macrophages, CD169 marks the perifollicular zone macrophages, CD19 marks B cells in the follicles. B cells in the follicle are also positive for FcγRIIb. Figures are representative of n = 3 spleens from different donors.