Intracellular FV and AAT levels in WT, Lman1^gt1/gt1, Mcfd2^−/−, and DKO mice. (A) Comparison of liver FV and AAT levels. Equal amounts of cell lysates (20 µg) from male mouse liver of the indicated genotypes were immunoblotted for FV, AAT, and TRAPα, an ER-resident protein. (B) Comparison of liver AAT levels in male and female mice. Equal amounts of cell lysates (20 µg) from mouse liver of the indicated genotypes and genders were immunoblotted for AAT and β-actin. A WT plasma sample was loaded in the last lane as a positive control.