FV, FVIII, and AAT levels in WT, Lman1^gt1/gt1, Mcfd2^−/−, and DKO mice. (A) Plasma was collected from blood drawn by cardiac puncture from mice euthanized at ∼6 months of age. FV antigen and FVIII activity levels in 3 deficient mice were normalized against average WT levels. (B) Plasma AAT levels were measured by ELISA, and comparisons were made within male and female groups of mice. Error bars indicate SD. *P < .05, **P <. 01, ***P < .001.