Selectin-interacting mouse leukocytes from bone marrow or peripheral blood are mature neutrophils. (A-E) Scatter profiles of the indicated mouse leukocyte populations. A gate (red circle in each panel) was set around cells expressing high levels of Ly6G. (F-K) Expression levels of the indicated surface protein in the gated populations. The colored histograms numbered 1 to 5 in each panel correspond to the cells in A-E. (L) Rolling velocities of cells from the indicated population on E-selectin with or without co-immobilized ICAM-1 in the presence or absence of anti-ICAM-1 mAb, the Src family kinase inhibitor PP2, or its inactive analog PP3. (M) Percentages of cells from the indicated population rolling, arrested and round, or arrested and spread on co-immobilized E-selectin, ICAM-1, and CXCL1 in the presence of the chemokine receptor inhibitor PTx or its solvent control dimethyl sulfoxide (DMSO). The histograms in A-K are representative of 5 independent experiments. The data in L and M represent the mean ± SEM from 5 experiments, with 5 mice in each experimental group. *P < .05 for rolling velocity; #P < .05 for percentage of rolling cells compared with that in WT, as determined by unpaired Student t test.