Circulating total and bcl2-JH cell-free DNA in FL at diagnosis. cfDNA was extracted from 400 to 900 µL of plasma. (A) The Eqg per mL of plasma was evaluated by ddPCR quantification of a reference gene (ANKRD30B gene, centromeric localization on chromosome 18). Each spot represents a patient. The vertical logarithmic scale highlights the large interpatient variability in the total amounts of cfDNA. (B) The number of circulating bcl2-JH segments were quantified by ddPCR in 26 patients. The red circles correspond to the number of bcl2-JH allele copies and the blue circles to the number of Eqg.