ATG-binding and ATG-mediated cytotoxicity of CB and BM cells. ATG-binding and ATG-mediated cytotoxicity in cells from CB (n = 3) and BM grafts (n = 3). (A) Binding of ATG to graft cells after exposure to 0, 1, 10, 50, and 100 μg/mL rabbit ATG, as evaluated using a FITC-labeled donkey anti-rabbit antibody (total cells, T cells, B cells, NK cells, and monocytes). (B) CDC is evaluated as the percentage of 7-AAD+ immune cells after exposure to 0, 1, 10, 50, and 100 μg/mL rabbit ATG (total cells, T cells, B cells, NK cells, and monocytes). ATG-mediated ADCC is evaluated as the percentage of 7-AAD+ immune cells after exposure to 0, 1, 10, and 100 μg/mL rabbit ATG. (C) NK ADCC (total cells, T cells, B cells, and monocytes). (D) Neutrophil ADCC (total cells, T cells, B cells, and NK cells). (E) Monocyte/macrophage ADCP is evaluated as the amount of remaining target cells/1000 beads after exposure to 0, 1, 10, and 100 μg/mL rabbit ATG (total cells, T cells, B cells, and NK cells). No significant differences were observed. n.a., not applicable.