Figure 1.
Anti-CD3ε PEBLs block surface CD3 expression. (A) Flow cytometric dot-plots illustrate surface CD3 downregulation in Jurkat by anti-CD3ε PEBLs compared with cells transduced with GFP alone (“Control”) or SEKDEL. (B) Surface CD3 expression in Jurkat transduced with the indicated constructs. Bars show a mean of 2 to 3 experiments for KEDL, SEKDEL, and PEBL 2,4,5,8,9,11, or individual results for the remainder. (C) Intracellular or surface expression of PEBL-derived anti-CD3ε scFv in Jurkat. Bars show a mean of 2 to 3 experiments for PEBL 2,4,8,9,11, or individual results for the remainder. (D) Flow cytometric histograms illustrate CD3 expression in peripheral blood T cells transduced with anti-CD3ε SEKDEL or PEBLs, relative to that of lymphocytes transduced with GFP alone, 8 to 13 days posttransduction.

Anti-CD3ε PEBLs block surface CD3 expression. (A) Flow cytometric dot-plots illustrate surface CD3 downregulation in Jurkat by anti-CD3ε PEBLs compared with cells transduced with GFP alone (“Control”) or SEKDEL. (B) Surface CD3 expression in Jurkat transduced with the indicated constructs. Bars show a mean of 2 to 3 experiments for KEDL, SEKDEL, and PEBL 2,4,5,8,9,11, or individual results for the remainder. (C) Intracellular or surface expression of PEBL-derived anti-CD3ε scFv in Jurkat. Bars show a mean of 2 to 3 experiments for PEBL 2,4,8,9,11, or individual results for the remainder. (D) Flow cytometric histograms illustrate CD3 expression in peripheral blood T cells transduced with anti-CD3ε SEKDEL or PEBLs, relative to that of lymphocytes transduced with GFP alone, 8 to 13 days posttransduction.

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