Establishment of iPSCs with different HLA genotypes from the monocytes of a patient with acquired AA. (A) HLA-A*24:02–lacking cells in the different lineages of cells. Histograms of HLA-A*24:02-expressing cells in different lineages of the patient (green lines) and a healthy individual (blue lines) are shown. (B) HLA-B4002 expression in monocyte fractions of the patient. Three different populations including A24⁺B4002⁺ (WT), A24⁺B4002⁻ (B*40:02^mut), and A24⁻B4002⁻ (6pLOH⁺) monocytes are shown. (C) Results of TaqMan PCR on 14 iPSC clones showing 10 6pLOH⁺ iPSC clones (red dots) and 4 6pLOH⁻ iPSC clones (green dots) that include 1 B4002⁻ clone; the blue dots represent control DNA. The x-axis and y-axis represent the amplified product amounts of B*40:02 and B*35:01, respectively. (D) Next-generation sequencing findings for B4002⁻ iPSC clone G2 showing a start loss mutation in B*40:02. The results of the HLA-B allelic sequencing of WT iPSC clone E3 is shown as a control. The green bar represents the start loss mutation locus of the B*40:02^mut clone. (E) The percentages of WT, B*40:02^mut, and 6pLOH⁺ iPSC clones are shown.