KDM3A upregulates MALAT1, which contributes to glycolytic activation via HIF-1α stabilization under hypoxia in myeloma cells. (A) Number of probes under indicated conditions: blue area shows region of genes upregulated under hypoxia and regulated by KDM3A only under hypoxia (cell line: U266, data uploaded at GSE96858). (B) qRT-PCR of MALAT1 for indicated cell lines cultured in normoxia or hypoxia (1% O2) for 48 hours. Bars represent mean ± 95% CI of 3 independent experiments. **.001 ≤ P < .01; ***P < .001. Student t test was used to test for significance. (C) qRT-PCR of MALAT1 for indicated cell lines transiently transduced with siKDM3A#1, #2, and control scrambled siRNA (Scr) and cultured in normoxia or hypoxia (1% O2) for 72 hours. Bars represent mean ± 95% CI of 3 independent experiments. *.01 ≤ P < .05; **.001 ≤ P < .01; ***P < .001. Student t test was used to test for significance. (D) qRT-PCR of MALAT1 for KMS-11 cell line transiently transduced with siMALAT1 or control Scr and cultured in normoxia or hypoxia (1% O2) for 48 hours. Bars represent mean ± 95% CI of 3 independent experiments. ***P < .001. Student t test was used to test for significance. (E) Western blot analysis of HIF-1α for KMS-11 cell line transiently transduced with siMALAT1 or control Scr and cultured in normoxia or hypoxia (1% O2) for 48 hours. (F) qRT-PCR of PFKFB3, PFKFB4, and SLC2A1 for KMS-11 cell line transiently transduced with siMALAT1 or control Scr and cultured in normoxia or hypoxia (1% O2) for 48 hours. Bars represent mean ± 95% CI of 3 independent experiments. **.001 ≤ P < .01; ***P < .001. Student t test was used to test for significance.
