Mice with a targeted CRISPR mutation in the ankyrin binding site of β spectrin are resistant to malaria.Survival (Ai) and parasitemia (Aii) of WT and Sptb^{D1781R/D1781R} mice infected with 1 × 10⁴P chabaudi parasites. (B) IVET assay results (as described in Figure 4) using Sptb^{D1781R/D1781R}- and WT-labeled erythrocytes. Percentage of infected cells (ratio to +/+) (Bi) and remaining cells (ratio to +/+) (Bii). Mean and standard error of the mean are indicated. The 1-sample t test was used to calculate P values for ratios; Mann-Whitney U test was used otherwise. *P < .05, **P < .01, ***P < .001.