Figure 6.
Figure 6. Hematopoietic factor and adhesion molecule production by LCs and SVECs. (A) The GEA values (as described in Figure 2A) of genes encoding factors supporting hematopoiesis. (B) RT-qPCR analysis of IL15, IL34, KITLG (SCF), CXCL12, JAG1, and JAG2, as described in Figure 2H. (C) The GEA values of genes encoding adhesion molecules. (D) RT-qPCR analysis of VCAM1, ESAM, and CDH11 (OB-cadherin), as described in Figure 2H. (E) Representative FACS histogram of SCF cell surface expression by LCs and SVECS. (F) Representative FACS histogram of intracellular IL-33 in fixed LCs and SVECS. Gate M1: IL-33+ based on IgG. Gate M2: IL-33high. (G) The percentage of IL-33high cells (Gate M2) relative to total LCs and SVECs. Data are shown as mean ± SD, n = 3 (triplicate analyses). *P = .02271 by paired Student t test. (H) Representative FACS histograms of VCAM1. (I) Percentage of VCAM1+ cells in SVECs and LCs. Data are shown as mean ± SD, n ≥ 3. **P = .007786 by 2-sample Student t test.

Hematopoietic factor and adhesion molecule production by LCs and SVECs. (A) The GEA values (as described in Figure 2A) of genes encoding factors supporting hematopoiesis. (B) RT-qPCR analysis of IL15, IL34, KITLG (SCF), CXCL12, JAG1, and JAG2, as described in Figure 2H. (C) The GEA values of genes encoding adhesion molecules. (D) RT-qPCR analysis of VCAM1, ESAM, and CDH11 (OB-cadherin), as described in Figure 2H. (E) Representative FACS histogram of SCF cell surface expression by LCs and SVECS. (F) Representative FACS histogram of intracellular IL-33 in fixed LCs and SVECS. Gate M1: IL-33+ based on IgG. Gate M2: IL-33high. (G) The percentage of IL-33high cells (Gate M2) relative to total LCs and SVECs. Data are shown as mean ± SD, n = 3 (triplicate analyses). *P = .02271 by paired Student t test. (H) Representative FACS histograms of VCAM1. (I) Percentage of VCAM1+ cells in SVECs and LCs. Data are shown as mean ± SD, n ≥ 3. **P = .007786 by 2-sample Student t test.

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