TLR2 inhibits ferroportin expression independent of hepcidin. Hepcidin gene silencing in THP-1 cells was achieved by lentiviral-based shRNA transduction. Scramble shRNA (Sh Scram) was used as a negative control. (A) Hepcidin expression in Sh Scram and hepcidin-specific shRNA (Sh HAMP) silenced THP-1 cells. (B) Surface ferroportin expression in Sh Scram and Sh HAMP THP-1 cells after Pam3CSK4 or LPS treatment for 24 hours. All data were from 3 independent experiments.