Fibrinolytic activity of exosomes and MVs on a plasma clot. The analysis was performed on MVs from MDA-MB-231 cells (A) and exosomes from MDA-MB-231 cells (B). Each experiment was repeated 3 times with different samples. The plasma clot was formed by incubating human platelet-poor plasma with thrombin. Then, exosomes and MVs (30 μg of protein) were added to the top of clot, followed by plasminogen (0.15 μM), with or without inhibitor (ε-AcA at 100 mM or Apro at 2.2 μM). A positive control was included, in which plasminogen (0.15 μM) and tPA (25 nM) were added directly to the freshly formed clot. The OD was measured at 350 nm every 20 minutes for 18 hours at 37°C. Results are presented as mean ± SEM (n = 3 independent samples). The percentage of clot retention is shown for MVs (Ai) and exosomes (Bi) from MDA-MD-231 cells. A representative photograph of the experiment is shown for MVs (Aii) and exosomes (Bii) from MDA-MD-231 cells. ****P ≤ .0001, Mann-Whitney U test (1 tailed).