Restriction of Fe-S cluster biogenesis increases expression of the acetyltransferase MEC17 and induces hyperacetylation of tubulin. (A) LPS/IFN-γ activation of RAW264.7 cells increased acetylated α-tubulin (ac-tubulin) levels. (B-C) Levels of the cytosolic acetyltransferase MEC17 and tubulin acetylation increased in response to knockdown of FXN (B) or ISCU (C). (D) Schematic illustrating how restriction of the Fe-S cluster biogenesis machinery may increase (blue) or decrease (red) key metabolites and proteins important in metabolic and epigenetic remodeling.