Western blot analysis of EV samples of 2 patients. Western blot analysis of EV samples of 2 patients (PT 1 and PT 2; which are samples of EVs isolated from patients 10 and 12, respectively) and EVs from 2 age-matched controls (labeled CT 1 and CT 2) probed using antibodies to the indicated proteins. In concordance with proteomics, the patients’ EVs had an increase in abundance of AHSP and catalase when compared with control samples, and markedly decreased haptoglobin and hemopexin protein levels. An example of Coomassie-stained gel is shown to demonstrate the accuracy of loading.