FXIII_plasmainfusion into F13a^−/−mice rescues thrombus weight.F13a^+/+, F13a^+/−, and F13a^−/− mice infused with vehicle and F13a^−/− mice infused with FXIII-A₂B₂ were subjected to the IVC venous thrombosis model. (A) Thrombi were harvested after 24 hours and weighed. Each dot represents a separate mouse. (B) Correlation between FXIII-A level in plasma (measured by western blot and densitometry, shown as relative band intensity, arbitrary fluorescence units) and thrombus weights for F13a^+/+, F13a^+/−, F13a^−/− (from panel A), and FXIII-A₂B₂-infused mice. Each dot represents a separate mouse; F13a^+/+ (green closed circles), F13a^+/− (blue half circles), F13a^−/− (red open circles) mice, and FXIII-A₂B₂-infused F13a^−/− mice targeting 100% (green triangles), 50% (blue triangles), 25% (pink triangles), and 10% (orange triangles) FXIII-A₂B₂. (C) Representative western blots showing FXIII-A in plasma immediately after infusion of 50% FXIII-A₂B₂ (plasma final, top), and in plasma (middle) and platelets (bottom) 24 hours after infusion and ligation. Note that infused human FXIII-A₂B₂ is more strongly detected than mouse.