Figure 6.
![Figure 6. Pemetrexed influx kinetics. Influx of 0.05 to 5.0 µM [3H]pemetrexed was assessed in cells transfected with either PCFT-WT or the N411K mutant. Data are best fit to the Michaelis-Menten equation V = Vmax[S]/(Kt + [S]), where [S] is extracellular substrate concentration, Vmax is the maximum transport rate, and Kt is the concentration at which influx is one half of maximum. Data shown are the means ± SEM from 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/1/10.1182_bloodadvances.2017012690/3/advances012690f6.jpeg?Expires=1723188346&Signature=CYaUxacKFQdjnGmPF2TDuwRHYaBfae2sE2hc8uWmX6nLoRV6tIh8FvBJMG4NiDDkCHwIwb8VLG4rkQ2PYKqIElFH-mCbNjUKGTR1JP0QejhwslF54KVHCo6PFpUTbWpMvvbXb1FB2ikYkw8SS~7kRRlhpy4xKDzr1G3fAQBwrZYq7ZYCVVJ6Dq9lsRleFndoC5sMLNCpGMLMu3Uh0rJmj58~POgSPkR6Q8CT~YE7iPk8yEu25EyiP8Xy8BGuEq-z6Ou1iCrOAjV-MyHgTcFVtrXa~jXaSAOFqA8GNQ1t8gQUuEdEv~7O8sSvt4nVKdx6WTmWhX1s4br~nKvtIfJDSg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pemetrexed influx kinetics. Influx of 0.05 to 5.0 µM [3H]pemetrexed was assessed in cells transfected with either PCFT-WT or the N411K mutant. Data are best fit to the Michaelis-Menten equation V = Vmax[S]/(Kt + [S]), where [S] is extracellular substrate concentration, Vmax is the maximum transport rate, and Kt is the concentration at which influx is one half of maximum. Data shown are the means ± SEM from 3 independent experiments.
