Figure 3.
![Figure 3. Effects of sulfhydryl modifications of the N411C PCFT mutant. Cells harboring the N411C PCFT mutation were treated with 3 mM MTSES, 3 mM MTSET, or 0.5 mM MTSEA-biotin for 30 min at pH 7.4 and RT followed by assessment of [3H]MTX influx. Data are percentages of untreated cells ± SEM, from the average of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/1/10.1182_bloodadvances.2017012690/3/advances012690f3.jpeg?Expires=1724990863&Signature=xfTO1M3EN470sMPZRTc1-EG39IHwDaTvjBtD2HhiU4WUKAZmd0kdFEIa7BMb6jO~Elnqv7FcAQEVkPDNNnEJ9PUUoDfkRA9GKV3CRlIH1WNe7~D3~shCJdwh8x~CrqnubCc59411-TNmvPpAMfjcMfiylLwUj4qnsye5YHnb8o7jKdlrrIMpXEtEOnZyAIuxVyosmejZA8QjHSwiih7mIX9jAFH95D4E3Uf657FkywWwwg05vaIHwN-ZaUgc3tm~xIRnh~5Uv0vjkvIhVSpLuJwjxzZ0fA3b~VC6~piU85hB~P~jTiXpskGlIsG6Zw10OkUPORSp9spzzV9mEybmPQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of sulfhydryl modifications of the N411C PCFT mutant. Cells harboring the N411C PCFT mutation were treated with 3 mM MTSES, 3 mM MTSET, or 0.5 mM MTSEA-biotin for 30 min at pH 7.4 and RT followed by assessment of [3H]MTX influx. Data are percentages of untreated cells ± SEM, from the average of 3 independent experiments.
