Figure 2.
![Figure 2. Structural requirement at the Asn411 residue. (A) Expression at the cell surface (upper row), and in the crude membrane fraction (middle row), of the Asn411 mutants. β-Actin was the internal control (lower row). The blot is representative of 3 independent experiments. (B) Influx of 0.5 and 50 μM [3H] MTX was measured in HeLa R1-11 cells transfected with a variety of substitutions at the Asn411 residue. Data are the averages ± SEM of 3 independent experiments. *P < .01; **P < .0001, in comparison with PCFT-WT activity.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/1/10.1182_bloodadvances.2017012690/3/advances012690f2.jpeg?Expires=1723188438&Signature=tRuFsgg4BfK3ZDFSGXsYLMpM6fMeA9n6RNSb2O32FkuTmjgFvigToHwkTrVv8vMNayunOWkTWUNScwiSqPVoXzECkbPCRTTsDsxFjK2FDOhXRTx9hMIjyFzdOHUVy7pV~-j4Mizlj-i17Rw9-IqxT1mck2fxQ6Fp3CNebU0783Lo7F42ZE~rH4~kNTczIy2ffaSvDkVvZAYtntVD2Fe3c~S~8VE5ohgYyCfhj5rPoCjtLRxbhuPMkte02K7yfATNNT6d3JiOoSO~zlegizNnuO3elap83al0oDbHkdK1NNFSYv-8iHS-pRa1Owb3CvJK7S9puZqWWQlnZ48xFKpfvA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Structural requirement at the Asn411 residue. (A) Expression at the cell surface (upper row), and in the crude membrane fraction (middle row), of the Asn411 mutants. β-Actin was the internal control (lower row). The blot is representative of 3 independent experiments. (B) Influx of 0.5 and 50 μM [3H] MTX was measured in HeLa R1-11 cells transfected with a variety of substitutions at the Asn411 residue. Data are the averages ± SEM of 3 independent experiments. *P < .01; **P < .0001, in comparison with PCFT-WT activity.
