Figure 1.
![Figure 1. Expression and function of the N411K PCFT mutant. (A) Influx of 0.5 μM [3H] MTX was assessed over 1 min at 37°C in RFC-/PCFT-null HeLa R1-11 cells transfected with wild-type PCFT, the N411K mutant, or vector control. Data are indicated as percentage of PCFT-WT activity and are the average of 3 independent experiments ± SEM. (B) Expression at the cell surface is depicted in the upper row. PCFT expression in the crude membrane preparation is depicted in the lower row. The western blot is representative of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/1/10.1182_bloodadvances.2017012690/3/advances012690f1.jpeg?Expires=1730434535&Signature=lr5dhusS~xwnwfuIqcozBOHiS7WpV53kcyxOLSw8cuCfHCPJaIAjEvwQ1231DMr2MzR7R90NWvLGsSvow7kVuJ0cnRu9PRcyIX3GiW63N3lvebA8b1tr9qu04b1KwN653HXr5YJ9tNavVqLKR3V2KFSjEDRRMeKn-0XS8a6618jZkfbPqCUBqKScfiBlvF0qQotL8XF7dzJHMI0wGHu4V7fASf5wiB8MkBlNE2wZKPyUsY3WqV0Pfw8C-v0ll1yB3nqrqDOQwtXutmWRD~~qz7QRciRo3AScDB4viGjsrvMdebBcK-CAYNa51sJRDrkZlW1btQRbw8O8u2ZfgcZ9cg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression and function of the N411K PCFT mutant. (A) Influx of 0.5 μM [3H] MTX was assessed over 1 min at 37°C in RFC-/PCFT-null HeLa R1-11 cells transfected with wild-type PCFT, the N411K mutant, or vector control. Data are indicated as percentage of PCFT-WT activity and are the average of 3 independent experiments ± SEM. (B) Expression at the cell surface is depicted in the upper row. PCFT expression in the crude membrane preparation is depicted in the lower row. The western blot is representative of 3 independent experiments.
