IL-12 and IL-15 induce a type 1 cytokine profile in ILC3s. Splenic NKp44⁺ ILC3s, cultured for 4 weeks with IL-2 and IL-7 or IL-12 and IL-15, were stimulated for 4 hours with K562 target cells or PMAi and analyzed for cytokine production. Flow cytometric analysis of IFNγ and TNFα expression in IL-12 and IL-15–treated cells (A-B) and quantification of IFNγ- and/or TNFα-expressing cells in the indicated conditions (C). N = 6 (3 HFL donors, 4 mice per donor). Red lines show mean and box-and-whiskers plot extend minimum (min) to maximum (max) with black lines representing median value. ns, P > .05; *P < .05 using paired Student t tests.