Adjuvants induce differences in the magnitude and durability of antibody responses. (A) Serum IgG-binding titers at week 26. (B) IgG-binding titers over time. Env and alum groups, top panel; emulsion groups, middle panel; pIC:LC and ISCOMs, bottom panel. Env + alum is shown as a benchmark; vaccine group geometric means are plotted; vertical dashed lines indicate immunizations. (C) Env IgG titer half-lives were calculated for each animal for the period between weeks 26 and 89. (D-E) Immunogenicity of clinically relevant adjuvant formulations in mice, vaccinated at weeks 0 and 3. Antibody titers are shown broken out at week 5 (D) or over a 44-week period (E). Horizontal bars indicate geometric means; *P < .05; **P < .01; ***P < .001 by the Kruskal-Wallis test in which the Env alone or Env + alum group (as indicated) were set as the control.