Impact of LMP1 expression on levels of proviability kinases in human BCL cell lines. (A) Representative experiments in which whole-cell lysates of the indicated cell lines were subjected to SDS-PAGE and western blotted for NIK, PIM2, LMP1, and actin as described in “Methods.” (B) Relative PIM2 protein levels (presented as mean ± SE from 3 independent experiments as in A), with PIM2 levels in SKW6.4 cells set as 1. (C) Relative NIK protein levels (presented as mean values ± SE from 3 independent experiments as in A), with NIK levels in SKW6.4 cells set as 1. Statistical significance of differences was calculated as described in “Methods”; *P < .006.