![Figure 2. Effect of LMP1 expression upon TRAF3 availability to bind membrane CD40 or BAFFR in human BCL lines. (A-C) Western blots representative of 3 similar experiments. CD40 was immunoprecipitated from lysates of the indicated BCL-derived lines as described in “Methods.” Immunoprecipitates (IP) or whole-cell lysates (WCL) were subjected to SDS-PAGE and western blotting with antibodies specific for the indicated proteins. (D) DAUDI or SKW6.4 cells were untreated (left two lanes) or treated for 2 hours with 50 ng/mL recombinant human BAFF (rBAFF), after which BAFFR was immunoprecipitated from cell lysates. (E) Relative levels (mean ± standard error [SE]) of BAFFR-associated TRAF3 from 3 independent experiments. Statistical significance of differences was calculated as described in “Methods”; *P < .0008.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/1/27/10.1182_bloodadvances.2017009670/3/advances009670f2.jpeg?Expires=1724961728&Signature=fBE0n5k6NN8MP754WKP8bcFKQ4Qcgj~QjmMPiyeCwiUy-3bKIJsut1q92ZpsLDfJgO6k3y5HvIOTtIVlhmGdU1P7~~vz2kzVsm5bw8y76syCuJec3HK3dDb8ylYhYhq2uXXJMHIDmB-IFRLlnLwCYhK-dMo9hWhmwTAH8Bu1iyKxg6rL0PuzNzmG38RwAiJTFUnEPK4t-pd-FKBFboqfifZ6cquobJ2YSXNMiVGVZnvzb-a9~55~rHhPWOBq3Gt4~1QoMTg9XhBzELV6QQtDo3rj3ZHhYS8r1Oi~b~mcDD1z2iNOtEJbBfWRkNgQYgFUo0LgkuJg1LIC2mP4sO8eUQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of LMP1 expression upon TRAF3 availability to bind membrane CD40 or BAFFR in human BCL lines. (A-C) Western blots representative of 3 similar experiments. CD40 was immunoprecipitated from lysates of the indicated BCL-derived lines as described in “Methods.” Immunoprecipitates (IP) or whole-cell lysates (WCL) were subjected to SDS-PAGE and western blotting with antibodies specific for the indicated proteins. (D) DAUDI or SKW6.4 cells were untreated (left two lanes) or treated for 2 hours with 50 ng/mL recombinant human BAFF (rBAFF), after which BAFFR was immunoprecipitated from cell lysates. (E) Relative levels (mean ± standard error [SE]) of BAFFR-associated TRAF3 from 3 independent experiments. Statistical significance of differences was calculated as described in “Methods”; *P < .0008.
