American Society of Hematology

Figure 1.

$Figure 1. Cytosolic kinases are activated by PIs and phosphorylate HSF1. (A) HSF1 S326 is phosphorylated in the cytoplasm. MM.1s and KMS18 cells were treated with 8 nM bortezomib for 9 hours followed by either total lysis or subcellular fractionation and equal cell number western blot analysis. Data are representative of 4 independent experiments. (B) Human kinome phosphoprotein microarray identifies bortezomib (BZ)–induced targets. MM.1s cells were treated with 8 nM bortezomib for 9 hours. Protein lysis and all subsequent steps were performed using the R&D Systems Human Phospho-Kinase Antibody Array as per manufacturer's instructions. Coordinate pairs and box colors are matched with their respective targets as follows: red, A7/8 = JNK1/2/3; orange, A13/14 = p53 (S392); purple, B9/10 = Akt1/2/3; blue, B13/14 = p53 (S46); black, C5/6 = HSP27; yellow, C15/16 = c-Jun; green, E13/14 = p27. Data are representative of 2 independent experiments.$

Cytosolic kinases are activated by PIs and phosphorylate HSF1. (A) HSF1 S326 is phosphorylated in the cytoplasm. MM.1s and KMS18 cells were treated with 8 nM bortezomib for 9 hours followed by either total lysis or subcellular fractionation and equal cell number western blot analysis. Data are representative of 4 independent experiments. (B) Human kinome phosphoprotein microarray identifies bortezomib (BZ)–induced targets. MM.1s cells were treated with 8 nM bortezomib for 9 hours. Protein lysis and all subsequent steps were performed using the R&D Systems Human Phospho-Kinase Antibody Array as per manufacturer's instructions. Coordinate pairs and box colors are matched with their respective targets as follows: red, A7/8 = JNK1/2/3; orange, A13/14 = p53 (S392); purple, B9/10 = Akt1/2/3; blue, B13/14 = p53 (S46); black, C5/6 = HSP27; yellow, C15/16 = c-Jun; green, E13/14 = p27. Data are representative of 2 independent experiments.

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